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Addgene

pGEX-MK3
(Plasmid #30188)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30188 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-KG
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 3200
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kcna3
  • Alt name
    Kv1.3
  • Alt name
    MK3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2400
  • GenBank ID
    NM_008418
  • Entrez Gene
    Kcna3 (a.k.a. Kca1-3, Kv1.3, Mk-3)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX-MK3 was a gift from Bruce Tempel (Addgene plasmid # 30188 ; http://n2t.net/addgene:30188 ; RRID:Addgene_30188)

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