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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 30173 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFPV27
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Backbone manufacturerRamakrishnan et al., 2000
- Backbone size w/o insert (bp) 4981
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Vector typeMycobacteria expression
Growth in Bacteria
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Bacterial Resistance(s)Hygromycin, 200 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMycobacterium Strong Promoter (MSP)
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SpeciesM. marinum
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Insert Size (bp)500
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MutationGFPmut3 allele has improved brightness.
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Tag
/ Fusion Protein
- GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer pFPV27-Fwd: GAATCGGTGGTTGTGGTGAT
- 3′ sequencing primer GFP-R (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was derived from pMSP12::GFP by interrupting the aph gene (removing a small ~300bp NsiI fragment) and inserting the gene for Hygromycin resistance.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGFPHYG2 was a gift from Lalita Ramakrishnan (Addgene plasmid # 30173 ; http://n2t.net/addgene:30173 ; RRID:Addgene_30173) -
For your References section:
Mycobacterium marinum Erp is a virulence determinant required for cell wall integrity and intracellular survival. Cosma CL, Klein K, Kim R, Beery D, Ramakrishnan L. Infect Immun. 2006 Jun . 74(6):3125-33. 10.1128/IAI.02061-05 PubMed 16714540