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pMXs-GLIS1
(Plasmid #30166)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30166 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMXs-gw
  • Backbone manufacturer
    Shinya Yamanaka
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GLIS1
  • Alt name
    FLJ36155
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1931
  • GenBank ID
    NM_147193
  • Entrez Gene
    GLIS1

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ cloning site att B1 (unknown if destroyed)
  • 3′ cloning site att B2 (unknown if destroyed)
  • 5′ sequencing primer pMX-S1811
  • 3′ sequencing primer pMX-AS3200
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Gateway cassette is from Invitrogen Corporation.
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H. Gateway casette is from Invitrogen Corporation.

IMPORTANT NOTE: This plasmid does not match the canonical GLIS1 sequence. For a more recent GLIS1 plasmid from the Yamanaka lab, please proceed to the following page: www.addgene.org/33274

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMXs-GLIS1 was a gift from Shinya Yamanaka (Addgene plasmid # 30166 ; http://n2t.net/addgene:30166 ; RRID:Addgene_30166)

  • For your References section:

    Direct reprogramming of somatic cells is promoted by maternal transcription factor Glis1. Maekawa M, Yamaguchi K, Nakamura T, Shibukawa R, Kodanaka I, Ichisaka T, Kawamura Y, Mochizuki H, Goshima N, Yamanaka S. Nature. 2011 . 474(7350):225-9. 10.1038/nature10106 PubMed 21654807
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