pET28b-AF0623
(Plasmid
#30164)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 30164 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET28b
- Backbone size w/o insert (bp) 5500
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAF0623
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Alt nameDNA ligase
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SpeciesArchaeoglobus fulgidus
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Insert Size (bp)1665
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Entrez Genelig (a.k.a. AF0623)
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Tag
/ Fusion Protein
- His (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7-term
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28b-AF0623 was a gift from SeWon Suh (Addgene plasmid # 30164 ; http://n2t.net/addgene:30164 ; RRID:Addgene_30164) -
For your References section:
ATP-dependent DNA ligase from Archaeoglobus fulgidus displays a tightly closed conformation. Kim do J, Kim O, Kim HW, Kim HS, Lee SJ, Suh SW. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 Jun 1. 65(Pt 6):544-50. 10.1107/S1744309109017485 PubMed 19478428