pCAX Itgb1-FLAG
(Plasmid #30153)

• Depositing Labs: Dennis Selkoe, Tracy Young-Pearse
• Publication: Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69.

Backbone

• Vector backbone: pCAX
• Backbone size w/o insert (bp): 1380
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: integrin beta 1
• Species: M. musculus (mouse)
• Insert Size (bp): 2500
• Entrez Gene: Itgb1 (a.k.a. 4633401G24Rik, CD29, Fnrb, Gm9863, gpIIa)
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry