pCAX APP C99
(Plasmid #30146)

• Depositing Labs: Dennis Selkoe, Tracy Young-Pearse
• Publication: Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69.

Backbone

• Vector backbone: pCAX
• Backbone size w/o insert (bp): 4731
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: APP C99
• Alt name: Amyloid Precursor Protien fragment
• Species: H. sapiens (human)
• Insert Size (bp): 297
• Mutation: C-terminal from BACE site of APP
• Entrez Gene: APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: BglII (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The insert contains an additional 83 amino acids of unknown composition on the n-terminus. It is unknown whether these amino acids affect plasmid function.