pCAX APP Swe/Ind
(Plasmid #30145)

• Depositing Labs: Dennis Selkoe, Tracy Young-Pearse
• Publication: Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69.

Backbone

• Vector backbone: pCAX
• Backbone size w/o insert (bp): 4731
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human APP 695 Swedish/Indiana mutation
• Alt name: amyloid precursor protein
• Species: H. sapiens (human)
• Insert Size (bp): 2000
• Mutation: Swedish and Indiana; K595N, M596L and V642F
• Entrez Gene: APP (a.k.a. AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, alpha-sAPP, preA4)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (destroyed during cloning)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Articles Citing this Plasmid:
  • 25 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry