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Addgene

Tet-O-FUW-Zic1
(Plasmid #30132)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 30132 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Tet-O-FUW
  • Backbone size w/o insert (bp) 8373
  • Vector type
    Mammalian Expression, Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zic1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1344
  • Entrez Gene
    Zic1 (a.k.a. ZIC, ZNF201)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer LNCX
  • 3′ sequencing primer WPRE-R
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Tet-O-FUW-Zic1 was a gift from Marius Wernig (Addgene plasmid # 30132 ; http://n2t.net/addgene:30132 ; RRID:Addgene_30132)

  • For your References section:

    Direct conversion of fibroblasts to functional neurons by defined factors. Vierbuchen T, Ostermeier A, Pang ZP, Kokubu Y, Sudhof TC, Wernig M. Nature. 2010 Feb 25. 463(7284):1035-41. 10.1038/nature08797 PubMed 20107439
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