pFastBac His6 MBP N10 TEV LIC cloning vector (4C)
(Plasmid #30116)

• Purpose: (Empty Backbone)
• Depositing Lab: Scott Gradia
• Publication: 4-series pFastBac LIC vectors (for insect cell expression) (unpublished)

Backbone

• Vector backbone: pFastBac
• Backbone size (bp): 5976
• Vector type: Insect Expression
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Tag / Fusion Protein:
  • His6-MBP-N10-TEV (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: LIC site (destroyed during cloning)
• 3′ cloning site: LIC site (destroyed during cloning)
• 5′ sequencing primer: MBP F (5'ggtcgtcagactgtcgatgaagcc)
• 3′ sequencing primer: LICBac R (5'caggttcagggggaggtgtg)

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is a LIC-adapted pFastBac vector. It uses the same PCR primer tags as with most of our other vectors, so one PCR product can be inserted into many different vectors at once.

This vector will add a His6-MBP-N10-TEV sequence to the N terminus of your protein. MBP may improve the solubility of your protein.

Add the following tags to your PCR primers:

LicV1 Forward Tag TACTTCCAATCCAATGCA

LicV1 Reverse Tag TTATCCACTTCCAATGTTATTA

Linearize this plasmid with SspI and gel purify the product, then T4-treat with dGTP. For the PCR product, T4-treat with dCTP.

For more information, please see our website:
http://qb3.berkeley.edu/qb3/macrolab/