pGEX-2T-IQGAP1
(Plasmid #30107)

• Depositing Lab: David Sacks
• Publication: Ho et al J Biol Chem. 1999 Jan 1. 274(1):464-70.

Backbone

• Vector backbone: pGEX-2T
• Backbone manufacturer: Amersham
• Backbone size w/o insert (bp): 4948
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IQGAP1
• Species: H. sapiens (human)
• Entrez Gene: IQGAP1 (a.k.a. HUMORFA01, SAR1, p195)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: pGEX5'

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry