HL 4212 (=HL 716 + pHL 1414)
(Plasmid
#30104)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 30104 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneColE (from pZ system) + AmpR
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Backbone manufacturerLim Lab (modified from Rolf Lutz and Hermann Bujard, 1997)
- Backbone size w/o insert (bp) 2000
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)HL 716
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepCon:fhlA::T7noRBS(900bps), pLlacO-1:rpoS::gfp
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SpeciesE. coli
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Insert Size (bp)3000
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Tag
/ Fusion Protein
- T7noRBS, GFP (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AatII (not destroyed)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer ctcatgagcggatacat atttgaa
- 3′ sequencing primer agctgatacc gctcgccgca gccgaacg (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HL 4212 (=HL 716 + pHL 1414) was a gift from Han Lim (Addgene plasmid # 30104 ; http://n2t.net/addgene:30104 ; RRID:Addgene_30104) -
For your References section:
Disruption of small RNA signaling caused by competition for Hfq. Hussein R, Lim HN. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28. 10.1073/pnas.1010082108 PubMed 21189298
Map uploaded by the depositor.
![](https://media.addgene.org/data/easy-thumbnails/data/79/87/019fd3cc-cf49-11e0-9003-003048dd6500.jpeg.940x940_q85_autocrop.png)