HL 3858 (=HL 716 + pHL 1304)
(Plasmid #30088)

• Depositing Lab: Han Lim
• Publication: Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 10.1073/pnas.1010082108. Epub 2010 Dec 28.

Backbone

• Vector backbone: ColE (from pZ system) + AmpR
• Backbone manufacturer: Lim Lab (modified from Rolf Lutz and Hermann Bujard, 1997)
• Backbone size w/o insert (bp): 2000
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): HL 716
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: pLtetO-1:rpoS::mCherry, pLlacO-1:ompC::gfp
• Species: E. coli
• Insert Size (bp): 2100
• Tag / Fusion Protein:
  • mCherry, GFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AatII (not destroyed)
• 3′ cloning site: ApaI (not destroyed)
• 5′ sequencing primer: ctcatgagcggatacat atttgaa
• 3′ sequencing primer: agctgatacc gctcgccgca gccgaacg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at or contact our distributors if you have any questions.