Skip to main content
Addgene

LacD_WT_pET21a
(Plasmid #29785)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29785 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET21a
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Tagatose 1,6-diphosphate aldolase
  • Alt name
    LacD
  • Species
    S. aureus (strain COL)
  • Insert Size (bp)
    978
  • Tag / Fusion Protein
    • His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LacD_WT_pET21a was a gift from SeWon Suh (Addgene plasmid # 29785 ; http://n2t.net/addgene:29785 ; RRID:Addgene_29785)

  • For your References section:

    Crystal structures of LacD from Staphylococcus aureus and LacD.1 from Streptococcus pyogenes: insights into substrate specificity and virulence gene regulation. Lee SJ, Kim HS, Kim do J, Yoon HJ, Kim KH, Yoon JY, Suh SW. FEBS Lett. 2011 Jan 21. 585(2):307-12. 10.1016/j.febslet.2010.12.038 PubMed 21192932
Related items:
Commonly requested with: