pET empty polycistronic destination vector (2D)
(Plasmid #29774)

• Purpose: (Empty Backbone)
• Depositing Lab: Scott Gradia
• Publication: 2-series polycistronic destination vectors (unpublished)

Backbone

• Vector backbone: pET
• Backbone size (bp): 2903
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: several (destroyed during cloning)
• 3′ cloning site: several (destroyed during cloning)
• 5′ sequencing primer: T7 forward
• 3′ sequencing primer: T7 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is an empty destination vector. It is a polycistronic vector that can express up to five genes at once. Genes must first be cloned into any of our 2-series transfer vectors, then subcloned into any of the five cassettes of the destination vector:

Cassette 1: BamHI/XbaI
Cassette 2: AsiSI/PspXI
Cassette 3: SbfI/AscI
Cassette 4: AdeI/NotI
Cassette 5: PacI/FseI

Please note that you must always insert your gene into the lowest cassette number first (e.g., if you're using cassettes 2 and 3, you must put your gene into cassette 2 first, then move on to cassette 3). You do not have to use all of the cassettes.

More information on this vector can be found through http://qb3.berkeley.edu/qb3/macrolab/