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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 29763 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBacPAK8
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 5500
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Vector typeInsect Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMYST1
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Alt nameMOF
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SpeciesH. sapiens (human)
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Insert Size (bp)1404
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GenBank IDBC037773
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Entrez GeneKAT8 (a.k.a. LIGOWS, MOF, MYST1, ZC2HC8, hMOF)
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Tag
/ Fusion Protein
- 2xHA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Bac1 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
A K215E mutation is present, it does not affect the function of the plasmid.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBacPAK8-2xHA-MOF was a gift from Joan Conaway (Addgene plasmid # 29763 ; http://n2t.net/addgene:29763 ; RRID:Addgene_29763) -
For your References section:
Subunit composition and substrate specificity of a MOF-containing histone acetyltransferase distinct from the male-specific lethal (MSL) complex. Cai Y, Jin J, Swanson SK, Cole MD, Choi SH, Florens L, Washburn MP, Conaway JW, Conaway RC. J Biol Chem. 2010 Feb 12. 285(7):4268-72. 10.1074/jbc.C109.087981 PubMed 20018852