pcDNA5FRT/Flag-OGT1
(Plasmid #29760)

• Depositing Lab: Joan Conaway
• Publication: Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72.

Backbone

• Vector backbone: pcDNA5FRT
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: OGT1
• Species: H. sapiens (human)
• Insert Size (bp): 3141
• Mutation: N764D and K1010E
• GenBank ID: NP_858059.1 NM_181672
• Entrez Gene: OGT (a.k.a. HINCUT-1, HRNT1, MRX106, O-GLCNAC, OGT1, XLID106)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The OGT1 insert contains the following mutations N764D and K1010E. These were present in the plasmid as it was described in the publication.