pAd/WT1-IRES-nAmCyan
(Plasmid #29756)

• Depositing Lab: Edward McCabe
• Publication: McCabe Lab Plasmids (unpublished)

Backbone

• Vector backbone: pAd/CMV/V5-DEST
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 36686
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Wilms tumor 1
• Alt name: WT1
• Species: H. sapiens (human)
• Insert Size (bp): 2300
• Mutation: No mutations; AmCyan is nuclear localized; WT1 and nAmCyan are separated by IRES; AmCyan from Clontech
• GenBank ID: BC032861
• Entrez Gene: WT1 (a.k.a. AWT1, GUD, NPHS4, WAGR, WIT-2, WT-1, WT33)
• Tags / Fusion Proteins:
  • IRES
  • Nuclear localization sequence
  • AmCyan (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attB1 (not destroyed)
• 3′ cloning site: attB2 (not destroyed)
• 5′ sequencing primer: attB1
• 3′ sequencing primer: V5

Resource Information

• A portion of this plasmid was derived from a plasmid made by: WT1 - Thermo Scientific/OpenBiosystems AmCyan - Clontech
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene distributes this plasmid in DH5a, but the depositing lab maintained the plasmid in OmniMAX™ 2 T1R (Invitrogen catalogue #C8540-03)