pAd/CMV/DAX1-IRES-nEGFP
(Plasmid #29752)

• Depositing Lab: Edward McCabe
• Publication: McCabe Lab Plasmids (unpublished)

Backbone

• Vector backbone: pAd/CMV/V5-DEST
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 36686
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1
• Alt name: DAX1
• Alt name: NR0B1
• Species: H. sapiens (human)
• Insert Size (bp): 2840
• Mutation: No mutations; Reporter is nuclear localized EGFP; DAX1 and nEGFP is separated with an IRES
• GenBank ID: BC011564
• Entrez Gene: NR0B1 (a.k.a. AHC, AHCH, AHX, DAX-1, DAX1, DSS, GTD, HHG, NROB1, SRXY2)
• Tags / Fusion Proteins:
  • IRES
  • Nuclear localization sequence
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ cloning site: attB1 (not destroyed)
• 3′ cloning site: attB2 (not destroyed)
• 5′ sequencing primer: attB1
• 3′ sequencing primer: V5

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Thermo Scientific/OpenBiosystems

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene distributes this plasmid in DH5a, but the depositing lab maintained the plasmid in OmniMAX 2 T1R (Invitrogen catalogue #C8540-03)