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pTH340-SUP45(1-242)-GalBD
(Plasmid #29740)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29740 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGBKT7
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 7267
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SUP45
  • Alt name
    eRF1, N- and M-domains
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    837
  • Mutation
    Codons 1-242 of the yeast SUP45 gene only
  • GenBank ID
    NM_001178491
  • Entrez Gene
    SUP45 (a.k.a. YBR143C, SAL4, SUP1, SUP47)
  • Tags / Fusion Proteins
    • Gal Activation domain (N terminal on backbone)
    • Myc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site PstI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH340-SUP45(1-242)-GalBD was a gift from Tobias von der Haar (Addgene plasmid # 29740 ; http://n2t.net/addgene:29740 ; RRID:Addgene_29740)
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