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Addgene

pTH671-SUP35-H348Q
(Plasmid #29732)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29732 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS313
  • Backbone size w/o insert (bp) 4904
  • Vector type
    Yeast Expression
  • Selectable markers
    HIS3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SUP35 gene encoding translation release factor 3 from S. cerevisiae
  • Alt name
    eRF3-H348Q
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    3195
  • Mutation
    H348Q mutant gene of SUP35 including genomic sequence 955 bp upstream of AUG and 185 bp downstream of stop codon.
  • GenBank ID
    NM_001180479
  • Entrez Gene
    SUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer M13f(-40)
  • 3′ sequencing primer M13r(-48)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH671-SUP35-H348Q was a gift from Tobias von der Haar (Addgene plasmid # 29732 ; http://n2t.net/addgene:29732 ; RRID:Addgene_29732)
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