UKC1620-SUP35wt
(Plasmid
#29730)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 29730 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS313
- Backbone size w/o insert (bp) 4904
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Vector typeYeast Expression
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Selectable markersHIS3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSUP35 gene encoding translation release factor 3 from S. cerevisiae
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Alt nameeRF3
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)3195
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MutationWild type gene including genomic sequence 955 bp upstream of AUG and 185 bp downstream of stop codon.
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GenBank IDNM_001180479
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Entrez GeneSUP35 (a.k.a. YDR172W, GST1, PNM2, SAL3, SUF12, SUP2, SUP36)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer M13f(-40)
- 3′ sequencing primer M13r(-48) (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
UKC1620-SUP35wt was a gift from Tobias von der Haar (Addgene plasmid # 29730 ; http://n2t.net/addgene:29730 ; RRID:Addgene_29730)
Map uploaded by the depositor.