pCR-Blunt II-TOPO-PTPRD
(Plasmid
#29705)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 29705 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCR-Blunt II-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 3519
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Vector typecloning vector
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameProtein tyrosine phosphatase receptor-type delta
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Alt namePTPRD
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Alt namePTP delta
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SpeciesH. sapiens (human)
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Insert Size (bp)5041
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Mutationnone
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GenBank IDBC106714
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Entrez GenePTPRD (a.k.a. HPTP, HPTPD, HPTPDELTA, PTPD, R-PTP-delta, RPTPDELTA)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer M13-Fwd, T7
- 3′ sequencing primer M13-Rev, SP6 (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byreceived from Open Biosystems, is a clone from the IMAGE Consortium
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCR-Blunt II-TOPO-PTPRD was a gift from Todd Waldman (Addgene plasmid # 29705 ; http://n2t.net/addgene:29705 ; RRID:Addgene_29705) -
For your References section:
Mutational inactivation of PTPRD in glioblastoma multiforme and malignant melanoma. Solomon DA, Kim JS, Cronin JC, Sibenaller Z, Ryken T, Rosenberg SA, Ressom H, Jean W, Bigner D, Yan H, Samuels Y, Waldman T. Cancer Res. 2008 Dec 15. 68(24):10300-6. 10.1158/0008-5472.CAN-08-3272 PubMed 19074898