FL-BCAN-lentiviral-GFP
(Plasmid
#29704)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 29704 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCDH1-MCS1-EF1-copGFP
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Backbone manufacturerSystem Biosciences
- Backbone size w/o insert (bp) 7544
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Vector typeLentiviral
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Selectable markerscopGFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBrevican
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Alt nameBCAN
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SpeciesH. sapiens (human)
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Insert Size (bp)2700
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Entrez GeneBCAN (a.k.a. BEHAB, CSPG7)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CMV (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Expresses GFP under the control of the EF1a promoter for monitoring delivery of
lentivector constructs into target cells
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FL-BCAN-lentiviral-GFP was a gift from Mariano Viapiano (Addgene plasmid # 29704 ; http://n2t.net/addgene:29704 ; RRID:Addgene_29704) -
For your References section:
Fibulin-3 is uniquely upregulated in malignant gliomas and promotes tumor cell motility and invasion. Hu B, Thirtamara-Rajamani KK, Sim H, Viapiano MS. Mol Cancer Res. 2009 Nov . 7(11):1756-70. 10.1158/1541-7786.MCR-09-0207 PubMed 19887559