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FL-fibulin-3-V5-lentiviral-GFP
(Plasmid #29703)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29703 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH1-MCS1-EF1-copGFP
  • Backbone manufacturer
    System Biosciences
  • Backbone size w/o insert (bp) 7544
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    copGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EFEMP1
  • Alt name
    fibulin-3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1479
  • GenBank ID
    BC098561
  • Entrez Gene
    EFEMP1 (a.k.a. ARCL1D, DHRD, DRAD, FBLN3, FBNL, FIBL-3, GLC1H, MLVT, MTLV, S1-5)
  • Tags / Fusion Proteins
    • V5 (C terminal on insert)
    • His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (destroyed during cloning)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer EF1a-R
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expresses GFP under the control of the EF1a promoter for monitoring delivery of
lentivector constructs into target cells

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FL-fibulin-3-V5-lentiviral-GFP was a gift from Mariano Viapiano (Addgene plasmid # 29703 ; http://n2t.net/addgene:29703 ; RRID:Addgene_29703)

  • For your References section:

    Fibulin-3 is uniquely upregulated in malignant gliomas and promotes tumor cell motility and invasion. Hu B, Thirtamara-Rajamani KK, Sim H, Viapiano MS. Mol Cancer Res. 2009 Nov . 7(11):1756-70. 10.1158/1541-7786.MCR-09-0207 PubMed 19887559
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