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Short fibulin-3-V5 pcDNA4
(Plasmid #29702)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29702 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA4.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EFEMP1
  • Alt name
    fibulin-3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1161
  • Mutation
    lacks N-terminus of EFEMP1 (amino acids 1-105)
  • Entrez Gene
    EFEMP1 (a.k.a. ARCL1D, DHRD, DRAD, FBLN3, FBNL, FIBL-3, GLC1H, MLVT, MTLV, S1-5)
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer BGH reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Short fibulin-3-V5 pcDNA4 was a gift from Mariano Viapiano (Addgene plasmid # 29702 ; http://n2t.net/addgene:29702 ; RRID:Addgene_29702)

  • For your References section:

    Fibulin-3 is uniquely upregulated in malignant gliomas and promotes tumor cell motility and invasion. Hu B, Thirtamara-Rajamani KK, Sim H, Viapiano MS. Mol Cancer Res. 2009 Nov . 7(11):1756-70. 10.1158/1541-7786.MCR-09-0207 PubMed 19887559
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