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Addgene

pTH490-tQSRTL
(Plasmid #29699)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29699 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS315
  • Backbone size w/o insert (bp) 5954
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tR(CCG)J; tQ(CUG)M; tT(CGU)K; tS(CGA)C; tL(GAG)G
  • Alt name
    Low abundance arginyl-; glutaminyl-; threonyl-; seryl- and leucyl tRNA genes.
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    1493

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site EclXI (not destroyed)
  • 5′ sequencing primer M13f (-40)
  • 3′ sequencing primer M13r (-48)
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

There is a single nucleotide (A) deletion in the sequence. This deletion does not affect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH490-tQSRTL was a gift from Tobias von der Haar (Addgene plasmid # 29699 ; http://n2t.net/addgene:29699 ; RRID:Addgene_29699)

  • For your References section:

    The role of tRNA and ribosome competition in coupling the expression of different mRNAs in Saccharomyces cerevisiae. Chu D, Barnes DJ, von der Haar T. Nucleic Acids Res. 2011 May 9. ():. 10.1093/nar/gkr300 PubMed 21558172
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