pTH652-RLuc/maxFLuc
(Plasmid #29698)

• Depositing Lab: Tobias von der Haar
• Publication: Chu et al Nucleic Acids Res. 2011 May 9. ():.

Backbone

• Vector backbone: pTH645-RLuc
• Backbone size w/o insert (bp): 7534
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Firefly Luciferase
• Alt name: FLuc
• Species: Photinus pyralis
• Insert Size (bp): 1659
• Mutation: All codons of the original FLuc gene were exchanged for those isoaccepting codons decoded by the most abundant tRNAs in yeast
• GenBank ID: AF043450

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Renilla Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry