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Addgene

pTH651-RLuc/minFLuc
(Plasmid #29697)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29697 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTH645-RLuc
  • Backbone size w/o insert (bp) 7534
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Firefly Luciferase
  • Alt name
    FLuc
  • Species
    Photinus pyralis
  • Insert Size (bp)
    1659
  • Mutation
    All codons of the original FLuc gene were exchanged for those isoaccepting codons decoded by the least abundant tRNAs in yeast
  • GenBank ID
    AF043450

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH651-RLuc/minFLuc was a gift from Tobias von der Haar (Addgene plasmid # 29697 ; http://n2t.net/addgene:29697 ; RRID:Addgene_29697)

  • For your References section:

    The role of tRNA and ribosome competition in coupling the expression of different mRNAs in Saccharomyces cerevisiae. Chu D, Barnes DJ, von der Haar T. Nucleic Acids Res. 2011 May 9. ():. 10.1093/nar/gkr300 PubMed 21558172
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