pTH644-CENBEVY
(Plasmid #29695)

• Depositing Lab: Tobias von der Haar
• Publication: Chu et al Nucleic Acids Res. 2011 May 9. ():.

Backbone

• Vector backbone: pRS316
• Backbone size w/o insert (bp): 4817
• Vector type: Yeast Expression
• Selectable markers: URA3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: bidirectional promoter cassette from pBEVY-U
• Alt name: P_ADH1/ P_TDH3 cassette
• Insert Size (bp): 1797

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: M13f (-40)
• 3′ sequencing primer: M13r (-48)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pBEVY-U obtained from EUROSCARF collection
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Miller, CA, Martinat, MA, Hyman, LE (1998) Nucleic Acids Res 26(15) 3577-3583. doi: 10.1093/nar/26.15.3577

Please note that there are minor mismatches between depositor's reference sequence and Addgene's quality control sequence. All differences are outside of the cloned gene, and do not affect the function of the plasmid.