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Addgene

pTH645-RLuc
(Plasmid #29694)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29694 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTH644_CENBEVY
  • Backbone size w/o insert (bp) 6598
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Renilla Luciferase
  • Alt name
    RLuc
  • Species
    Renilla muelleri
  • Insert Size (bp)
    948
  • GenBank ID
    AF043450

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaC1 (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer n/a
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that there are minor mismatches between depositor's reference sequence and Addgene's quality control sequence. All differences are outside of the cloned gene, and do not affect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTH645-RLuc was a gift from Tobias von der Haar (Addgene plasmid # 29694 ; http://n2t.net/addgene:29694 ; RRID:Addgene_29694)

  • For your References section:

    The role of tRNA and ribosome competition in coupling the expression of different mRNAs in Saccharomyces cerevisiae. Chu D, Barnes DJ, von der Haar T. Nucleic Acids Res. 2011 May 9. ():. 10.1093/nar/gkr300 PubMed 21558172
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