pAMV:nAChRgamma-eGFP
(Plasmid #29693)

• Depositing Lab: Henry Lester
• Publication: Pantoja et al Biophys J. 2009 Jan . 96(1):226-37.

Backbone

• Vector backbone: pBlueScriptKS(+)
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4928
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GFP tagged Mus musculus cholinergic receptor, nicotinic, gamma
• Alt name: Chrng
• Alt name: gamma-GFP
• Species: M. musculus (mouse)
• Insert Size (bp): 2300
• Mutation: eGFP inserted after position S380
• GenBank ID: NM_009604
• Entrez Gene: Chrng (a.k.a. Achr-3, Acrg)
• Tags / Fusion Proteins:
  • AMV promotor (N terminal on backbone)
  • poly(A) tail (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRV (unknown if destroyed)
• 3′ cloning site: BstXI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry