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Addgene

DsRed-Rab5-QL
(Plasmid #29688)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29688 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDsRed2-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RAB5A
  • Species
    canis familiaris
  • Insert Size (bp)
    699
  • Mutation
    Q79L
  • Tag / Fusion Protein
    • DSRed2 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bgl2 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer DsRed1-C 5'-AGCTGGACATCACCTCCCACAACG-3'
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Rab5a is from Cecilia Bucci through Addgene, and modified by our group
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DsRed-Rab5-QL was a gift from Edward De Robertis (Addgene plasmid # 29688 ; http://n2t.net/addgene:29688 ; RRID:Addgene_29688)

  • For your References section:

    Wnt signaling requires sequestration of glycogen synthase kinase 3 inside multivesicular endosomes. Taelman VF, Dobrowolski R, Plouhinec JL, Fuentealba LC, Vorwald PP, Gumper I, Sabatini DD, De Robertis EM. Cell. 2010 Dec 23. 143(7):1136-48. 10.1016/j.cell.2010.11.034 PubMed 21183076
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