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Addgene

pCS2 HRS-RFP
(Plasmid #29685)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29685 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2
  • Backbone size w/o insert (bp) 4096
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    hepatocyte growth factor-regulated tyrosine kinase substrate
  • Alt name
    HRS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2926
  • Entrez Gene
    HGS (a.k.a. HRS)
  • Tag / Fusion Protein
    • RFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (not destroyed)
  • 3′ cloning site SnaB1 (destroyed during cloning)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2 HRS-RFP was a gift from Edward De Robertis (Addgene plasmid # 29685 ; http://n2t.net/addgene:29685 ; RRID:Addgene_29685)

  • For your References section:

    Wnt signaling requires sequestration of glycogen synthase kinase 3 inside multivesicular endosomes. Taelman VF, Dobrowolski R, Plouhinec JL, Fuentealba LC, Vorwald PP, Gumper I, Sabatini DD, De Robertis EM. Cell. 2010 Dec 23. 143(7):1136-48. 10.1016/j.cell.2010.11.034 PubMed 21183076
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