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Addgene

mCherry-Pak3(60-113)/S74A/F84A-mCherry-C1
(Plasmid #29676)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29676 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    mCherry-C1
  • Backbone size w/o insert (bp) 4645
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Pak3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    165
  • Mutation
    aa60-113 only; S74A F84A
  • Entrez Gene
    PAK3 (a.k.a. ARA, MRX30, MRX47, OPHN3, PAK-3, PAK3beta, bPAK, beta-PAK)
  • Tags / Fusion Proteins
    • mCherry (N terminal on backbone)
    • mCherry (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer sequence
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-Pak3(60-113)/S74A/F84A-mCherry-C1 was a gift from Ryohei Yasuda (Addgene plasmid # 29676 ; http://n2t.net/addgene:29676 ; RRID:Addgene_29676)

  • For your References section:

    Local, persistent activation of Rho GTPases during plasticity of single dendritic spines. Murakoshi H, Wang H, Yasuda R. Nature. 2011 Mar 20. ():. 10.1038/nature09823 PubMed 21423166
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