pET23b-proMMP-7-E215Q
(Plasmid #29642)

• Depositing Lab: William Parks
• Publication: Ra et al J Biol Chem. 2009 Oct 9. 284(41):27924-32.

Backbone

• Vector backbone: pET23b
• Backbone manufacturer: EMD Biosciences
• Backbone size w/o insert (bp): 3700
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Plasmid provided in BL21 RIL cells "Not Codon Plus"
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: proMMP-7
• Alt name: Promatrilysin
• Species: H. sapiens (human)
• Mutation: E215Q Catalytically Inactive
• Entrez Gene: MMP7 (a.k.a. MMP-7, MPSL1, PUMP-1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

MMP7 sequence in this plasmid is suitable for expression in bacteria but NOT in mammalian cells, as the signal sequence is not included.

The MMP7 amino acid sequence starts at amino acid 18 when compared to GenBank accession number NP_002414.1. Note that a superfluous methionine corresponding to the start signal for translation is present at the N-terminus of the expressed protein

Please note that the His tag present in the vector is not in-frame with MMP-7