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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 29629 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDuet
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFUS
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Alt nameTLS
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Alt namefused in sarcoma
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Alt nametranslocated in liposarcoma
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SpeciesH. sapiens (human)
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Insert Size (bp)1578
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Entrez GeneFUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)
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Tags
/ Fusion Proteins
- GST (N terminal on insert)
- TEV (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST-TEV-FUS was a gift from Aaron Gitler (Addgene plasmid # 29629 ; http://n2t.net/addgene:29629 ; RRID:Addgene_29629) -
For your References section:
Molecular determinants and genetic modifiers of aggregation and toxicity for the ALS disease protein FUS/TLS. Sun Z, Diaz Z, Fang X, Hart MP, Chesi A, Shorter J, Gitler AD. PLoS Biol. 2011 Apr;9(4):e1000614. doi: 10.1371/journal.pbio.1000614. Epub 2011 Apr 26. 10.1371/journal.pbio.1000614 PubMed 21541367