pcDNA 3.2-FUS-1-413aa-V5
(Plasmid #29612)

• Depositing Lab: Aaron Gitler
• Publication: Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi: 10.1371/journal.pbio.1000614. Epub 2011 Apr 26.

Backbone

• Vector backbone: pcDNA3.2/V5-DEST
• Backbone size w/o insert (bp): 7711
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FUS-1-413aa
• Alt name: TLS
• Alt name: fused in sarcoma
• Alt name: translocated in liposarcoma
• Species: H. sapiens (human)
• Insert Size (bp): 1266
• Mutation: Contains FUS aa# 1-413
• Entrez Gene: FUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)
• Tag / Fusion Protein:
  • V5 (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: n/a

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry