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Addgene

pcDNA 3.2-FUS-1-526aa-V5
(Plasmid #29609)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29609 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.2/V5-DEST
  • Backbone size w/o insert (bp) 7711
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FUS
  • Alt name
    TLS
  • Alt name
    fused in sarcoma
  • Alt name
    translocated in liposarcoma
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1578
  • Entrez Gene
    FUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)
  • Tag / Fusion Protein
    • V5 (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA 3.2-FUS-1-526aa-V5 was a gift from Aaron Gitler (Addgene plasmid # 29609 ; http://n2t.net/addgene:29609 ; RRID:Addgene_29609)

  • For your References section:

    Molecular determinants and genetic modifiers of aggregation and toxicity for the ALS disease protein FUS/TLS. Sun Z, Diaz Z, Fang X, Hart MP, Chesi A, Shorter J, Gitler AD. PLoS Biol. 2011 Apr;9(4):e1000614. doi: 10.1371/journal.pbio.1000614. Epub 2011 Apr 26. 10.1371/journal.pbio.1000614 PubMed 21541367
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