Skip to main content
Addgene

416Gal-SV40NLS-FUS-YFP
(Plasmid #29595)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29595 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pAG416Gal-ccdB-EYFP
  • Backbone manufacturer
    Susan Lindquist (Addgene plasmid # 14219)
  • Backbone size w/o insert (bp) 8004
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Growth instructions
    ccdB Survival
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    NLS-FUS
  • Alt name
    TLS
  • Alt name
    fused in sarcoma
  • Alt name
    translocated in liposarcoma
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1602
  • Entrez Gene
    FUS (a.k.a. ALS6, ETM4, FUS1, HNRNPP2, POMP75, TLS, altFUS)
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on insert)
    • YFP (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To introduce the SV40 NLS to the N-terminus of FUS, we incorporated DNA
sequences encoding the SV40 NLS (PPKKKRKV), optimized for yeast translation (CCA CCA AAA
AAA AAA AGA AAA GTT) into the forward primer, following a start codon (ATG) and in frame
with FUS

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    416Gal-SV40NLS-FUS-YFP was a gift from Aaron Gitler (Addgene plasmid # 29595 ; http://n2t.net/addgene:29595 ; RRID:Addgene_29595)

  • For your References section:

    Molecular determinants and genetic modifiers of aggregation and toxicity for the ALS disease protein FUS/TLS. Sun Z, Diaz Z, Fang X, Hart MP, Chesi A, Shorter J, Gitler AD. PLoS Biol. 2011 Apr;9(4):e1000614. doi: 10.1371/journal.pbio.1000614. Epub 2011 Apr 26. 10.1371/journal.pbio.1000614 PubMed 21541367
Related items:
Commonly requested with: