pSpy
(Plasmid
#29588)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 29588 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLysE
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 4430
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSpy
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Alt namespheroplast protein Y
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Alt nameenvelope stress induced periplasmic protein
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SpeciesE.coli BL21(DE3)
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Insert Size (bp)420
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Mutationprocessed form of Spy starting at residue 24 (Removal of first 23aa of periplasmic sequence)
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GenBank IDAM946981
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Entrez Genespy (a.k.a. b1743, ECK1741, JW1732)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBR2
- 3′ sequencing primer pBR3 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSpy was a gift from Marc Fiedler (Addgene plasmid # 29588 ; http://n2t.net/addgene:29588 ; RRID:Addgene_29588)