pcDNA3/FLHIP1
(Plasmid
#29587)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 29587 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5446
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Vector typeBacterial Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHuntingtin-interacting protein 1
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SpeciesH. sapiens (human)
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Insert Size (bp)4425
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Entrez GeneHIP1 (a.k.a. HIP-I, ILWEQ, SHON, SHONbeta, SHONgamma)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA3/FLHIP1 was a gift from Theodora Ross (Addgene plasmid # 29587 ; http://n2t.net/addgene:29587 ; RRID:Addgene_29587) -
For your References section:
Huntingtin-interacting protein 1 is overexpressed in prostate and colon cancer and is critical for cellular survival. Rao DS, Hyun TS, Kumar PD, Mizukami IF, Rubin MA, Lucas PC, Sanda MG, Ross TS. J Clin Invest. 2002 Aug . 110(3):351-60. 10.1172/JCI15529 PubMed 12163454