mCherryC1-PIPKI{gamma}661
(Plasmid #29584)

• Depositing Lab: Anna Huttenlocher
• Publication: Lokuta et al Mol Biol Cell. 2007 Dec . 18(12):5069-80.

Backbone

• Vector backbone: mCherry C1
• Backbone size w/o insert (bp): 4673
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5 alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: phosphatidylinositol phosphate kinase type I gamma
• Alt name: PIPKI gamma 661
• Species: M. musculus (mouse)
• Insert Size (bp): 1986
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bgl II (not destroyed)
• 3′ cloning site: Kpn1 (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Ling et al., 2002 gift from Dr. Richard Anderson
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pmCherry-C1 was generated by PCR amplification of pRSET-B-mCherry (a generous gift from Dr. Roger Tsien) with primers containing a 5' NheI site and 3' BglII site and subcloning into pEGFP-C1 (Clontech) to replace the enhanced GFP (EGFP) sequence