pCDNA3.1(+) GFP-PIPKI{gamma}661-FLAG
(Plasmid #29583)

• Depositing Lab: Anna Huttenlocher
• Publication: Lokuta et al Mol Biol Cell. 2007 Dec . 18(12):5069-80.

Backbone

• Vector backbone: pCDNA3.1(+)
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5424
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: DH5 alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: phosphatidylinositol phosphate kinase type I gamma
• Alt name: PIPKI gamma 661
• Species: M. musculus (mouse)
• Insert Size (bp): 2715
• Mutation: N/A
• Tags / Fusion Proteins:
  • GFP (N terminal on insert)
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: Xho1 (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Ling et al., 2002 from Dr. Richard Anderson

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results identified a single nucleotide mismatch at bp#440 when compared to the full sequence information provided by the depositing laboratory.