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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 29581 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepMCSG10
- Backbone size w/o insert (bp) 5961
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMap3k1
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SpeciesH. sapiens (human)
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MutationConstitutively Active. Contains catalytic domain D874-end.
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Entrez GeneMAP3K1 (a.k.a. MAPKKK1, MEKK, MEKK 1, MEKK1, SRXY6)
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Tags
/ Fusion Proteins
- HIS6 (N terminal on backbone)
- GST (N terminal on backbone)
- TEV (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Ligation-independent-cloning.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Map3k1 was a gift from Dustin Maly (Addgene plasmid # 29581 ; http://n2t.net/addgene:29581 ; RRID:Addgene_29581) -
For your References section:
Sequence determinants of a specific inactive protein kinase conformation. Hari SB, Merritt EA, Maly DJ. Chem Biol. 2013 Jun 20;20(6):806-15. doi: 10.1016/j.chembiol.2013.05.005. 10.1016/j.chembiol.2013.05.005 PubMed 23790491