MKK2
(Plasmid #29580)

• Depositing Lab: Dustin Maly
• Publication: Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi: 10.1016/j.chembiol.2013.05.005.

Backbone

• Vector backbone: pMCSG10
• Backbone size w/o insert (bp): 5961
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MKK2
• Species: H. sapiens (human)
• Mutation: Constitutively active mutant; Starting Met removed; Deletion of EQQKKRLE, residues 48 to 55; S222D and S226D mutations present
• Entrez Gene: MAP2K2 (a.k.a. CFC4, MAPKK2, MEK2, MKK2, PRKMK2)
• Tags / Fusion Proteins:
  • HIS6 (N terminal on backbone)
  • GST (N terminal on backbone)
  • TEV (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-term

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene plasmid# 21194 was used as template for MKK2.

Ligation-independent-cloning.