pmCherryC1 Human WIP
(Plasmid #29573)

• Depositing Lab: Anna Huttenlocher
• Publication: Cortesio et al Mol Biol Cell. 2010 Jan 1. 21(1):186-97.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clonetech
• Backbone size w/o insert (bp): 4704
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: WASP-interacting protein
• Alt name: WIP
• Species: H. sapiens (human)
• Insert Size (bp): 1512
• Entrez Gene: WIPF1 (a.k.a. PRPL-2, WAS2, WASPIP, WIP)
• Tag / Fusion Protein:
  • mCherry (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Hind III (not destroyed)
• 3′ cloning site: EcoR1 (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Flag-hWIP was provided by Scott Weed (University of West Virginia)
• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Cloning of the pmCherry-C1 vector has been previously described Lokuta et al., 2007.
pmCherry-C1 was generated by PCR amplification of pRSET-B-mCherry (a generous gift from Dr. Roger Tsien) with primers containing a 5' NheI site and 3' BglII site and subcloning into pEGFP-C1 (Clontech) to replace the enhanced GFP (EGFP) sequence