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Addgene

SEMA4D (chick) pBLUE
(Plasmid #29568)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29568 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBLUESCRIPT (SK)
  • Backbone manufacturer
    stratagene
  • Backbone size w/o insert (bp) 2900
  • Vector type
    pBlue for ISH

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SEMA4D
  • Alt name
    Semaphorin 4D
  • Alt name
    Collapsin 4
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    3500
  • Mutation
    For ISH Sense: Not1 or Xho1/T3 Antisense: Kpn1 or EcoR1/T7
  • GenBank ID
    NC_006127
  • Entrez Gene
    SEMA4D (a.k.a. COLL-4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Xho1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SEMA4D (chick) pBLUE was a gift from Jonathan Raper (Addgene plasmid # 29568 ; http://n2t.net/addgene:29568 ; RRID:Addgene_29568)
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