pSG5-CRKL
(Plasmid #29560)

• Depositing Lab: Nora Heisterkamp
• Publication: Fioretos et al Oncogene. 1993 Oct . 8(10):2853-5.

Backbone

• Vector backbone: pSG5
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 4076
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CRKL
• Species: H. sapiens (human)
• Insert Size (bp): 1600
• GenBank ID: NM_005207
• Entrez Gene: CRKL

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SMAI (destroyed during cloning)
• 3′ cloning site: SMAI (destroyed during cloning)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Crkl pSG5. This construct encodes the human adapter protein Crkl. Crkl is a protein that contains one SH2 domain and two SH3 domains. It can become tyrosine phosphorylated by the c-Abl tyrosine kinase on residue Y207. Crkl is located on chromosome 22q11.21. Gene ID: 1399. HPRD: 03596. NM_005207.
The construct was made by Joanne ten Hoeve in 1992/1993.