hCent2-pEGFP-C1
(Plasmid #29559)

• Depositing Lab: Jeffrey Salisbury
• Publication: Salisbury et al Curr Biol. 2002 Aug 6. 12(15):1287-92.

Backbone

• Vector backbone: pEGFP-C1
• Backbone size w/o insert (bp): 4731
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Cent2
• Alt name: centrin 2
• Species: H. sapiens (human)
• Insert Size (bp): 516
• Entrez Gene: CETN2 (a.k.a. CALT, CEN2)
• Tag / Fusion Protein:
  • EGFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (unknown if destroyed)
• 3′ cloning site: XmaI (unknown if destroyed)
• 5′ sequencing primer: EGFP-C

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Centrin-2 sequence in this plasmid contains an additional serine at position 3 when compared to GenBank NP_004335.1