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pCDF1-ADAMTS18 (G312E)
(Plasmid #29553)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 29553 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDF1-EF1-MCS2-Puromycin
  • Backbone manufacturer
    Systems Biosciences Inc
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    ADAMTS18
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3640
  • Mutation
    changed glycine 312 to glutamic acid
  • GenBank ID
    NM_199355
  • Entrez Gene
    ADAMTS18 (a.k.a. ADAMTS21, KNO2)
  • Tag / Fusion Protein
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDF1-ADAMTS18 (G312E) was a gift from Yardena Samuels (Addgene plasmid # 29553 ; http://n2t.net/addgene:29553 ; RRID:Addgene_29553)

  • For your References section:

    Mutational and functional analysis reveals ADAMTS18 metalloproteinase as a novel driver in melanoma. Wei X, Prickett TD, Viloria CG, Molinolo A, Lin JC, Cardenas-Navia I, Cruz P, Rosenberg SA, Davies MA, Gershenwald JE, Lopez-Otin C, Samuels Y. Mol Cancer Res. 2010 Nov . 8(11):1513-25. 10.1158/1541-7786.MCR-10-0262 PubMed 21047771
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